Figure 4
From: Robust detection of undifferentiated iPSC among differentiated cells
Novel markers were suitable for detecting residual cells from three germ lineages. (a) Mesoderm and ectoderm lineage cells were successfully differentiated based on gene expressions. Representative image of differentiated cells and marker gene expressions of each lineage. bar: 500 um. (b) Marker genes were down-regulated in the course of differentiation in each lineage and (c) residual iPSC in each lineage. The relative expression levels were normalized by the amount of 18S rRNA in each sample. (d) smFISH detection of undifferentiated cells by ESRG gene expression with flow cytometry. Positive fraction specifically included stained, ESRG-positive cell. bar: 10 um.
