Figure 1
From: An integrative DNA barcoding framework of ladybird beetles (Coleoptera: Coccinellidae)

Amplification efficiency of new design primers. (a) Comparison amplification efficiency between standard polymerase chain reaction (PCR) and nested PCR for each primer pair; (b) standard PCR success rate of each pair of primers of different age classes; (c) nested PCR success rate of each pair of primers of different age classes.