Figure 4

Live/dead assay on spheroids grown with hanging drop. Representative CLSM images of hanging drop-based spheroids without MC (A,B) and with MC (C,D external regions; E,F internal regions) stained for live (calcein AM; 3 μM solution; in green) and dead (propidium iodide; 10 μM solution; in red) cells after 14 days. Bars = 100 µm.