Figure 7

qPCR analysis. Analysis of CD44, Ki-67, Vimentin and TGF-β1 genes in spheroids cultured in adherent conditions (ADH), Matrigel, hanging drop and with MC-enriched media (HD + MC). Data are normalized to GAPDH expression and are represented by the mean ± SEM (n > 3). Multiple t-test was used to compare the result in pairs (***p < 0.0005, **p < 0.005, *p < 0.05).