Table 4 Downstream signaling hits between cells expressing wild-type PBK and cells expressing p.Gly43Arg PBK (control and treatment) for 24 hours as analyzed by KAM-900P Antibody Microarray.
From: A novel loss-of-function mutation of PBK associated with human kidney stone disease
Target Protein Name | Phospho Site (Human) | Full Target Protein Name | Wild-type | p.Gly43Arg | %CFC (p.Gly43Arg from Wild-type) | ||
|---|---|---|---|---|---|---|---|
Globally Normalized intensity | %Error Range | Globally Normalized intensity | %Error Range | ||||
STAT2 | Y690 | Signal transducer and activator of transcription 2 | 12.07 | 25.00 | 3998.24 | 30.54 | 33020.71 |
CTNNB1 | Pan-specific | Catenin (cadherin-associated protein) beta 1 | 8899.32 | 36.86 | 39376.07 | 34.98 | 342.46 |
ABL1 | Pan-specific | Abelson proto-oncogene-encoded protein-tyrosine kinase | 370.84 | 1.81 | 1007.12 | 69.04 | 171.57 |
CDK2 | T160 | Cyclin-dependent protein-serine kinase 2 | 4985.72 | 4.41 | 13344.65 | 89.59 | 167.66 |
CDK1 | T14 | Cyclin-dependent protein-serine kinase 1 | 994.27 | 14.90 | 2458.99 | 97.52 | 147.32 |
CAV2 | Pan-specific | Caveolin 2 | 2203.31 | 14.29 | 4762.67 | 31.30 | 116.16 |
MKK4 | Pan-specific | MAPK/ERK protein-serine kinase 4 | 1560.23 | 13.09 | 3060.01 | 8.75 | 96.13 |
BRSK1 | T189 | BR serine/threonine-protein kinase 1 | 2885.11 | 30.13 | 5491.48 | 25.83 | 90.34 |
BCK2L7 | Pan-specific | Bcl2 homologous antagonist/killer | 581.21 | 6.27 | 1025.35 | 18.75 | 76.42 |
MAP3K7 | S439 | TGF-beta-activated protein-serine kinase 1 | 2880.44 | 11.14 | 5061.08 | 54.95 | 75.70 |
SIT | Y90 | Signaling threshold-regulating transmembrane adapter 1 | 3956.22 | 13.01 | 6810.59 | 16.99 | 72.15 |
CCNE1 | T395 | Cyclin E1 | 12311.81 | 20.41 | 20821.20 | 19.41 | 69.12 |
CHK1 | S280 | Checkpoint protein-serine kinase 1 | 6169.84 | 13.46 | 10126.81 | 0.50 | 64.13 |
CTNNB1 | S33 | Catenin (cadherin-associated protein) beta 1 | 17747.78 | 3.23 | 29076.53 | 7.81 | 63.83 |
CDK7 | Pan-specific | Cyclin-dependent protein-serine kinase 7 | 5430.82 | 19.64 | 8884.29 | 6.89 | 63.59 |
BLK | Y188 | B lymphoid tyrosine kinase | 1865.68 | 7.41 | 3028.97 | 14.49 | 62.35 |
CHK1 | S317 | Checkpoint protein-serine kinase 1 | 2336.62 | 11.57 | 3734.82 | 23.01 | 59.84 |
WNK1 | T2245 | Serine/threonine-protein kinase WNK1 | 1205.36 | 47.18 | 481.67 | 0.24 | −60.04 |
p53 | Pan-specific | Tumor suppressor protein p53 | 1547.83 | 26.94 | 602.34 | 18.41 | −61.09 |
MDM2 | S166 | Double minute 2 | 11394.35 | 5.37 | 4330.00 | 10.92 | −62.00 |
p70 S6K | Pan-specific | Ribosomal protein S6 kinase beta-1 | 1047.28 | 7.61 | 292.15 | 1.45 | −72.10 |
PDGFRa | Y754 | Platelet-derived growth factor receptor kinase alpha | 1927.40 | 21.94 | 527.39 | 17.82 | −72.64 |
