Figure 6
From: Label-free metabolic biomarkers for assessing valve interstitial cell calcific progression
Calcific nodules in osteogenic cultures were apoptotic in nature. (a) Representative Caspase immunostains (Caspase in red and nuclei in blue) of VIC cultures for days 1 through 28 under quiescent or osteogenic conditions on compliant or stiff substrates. (b) Average apoptosis index of nodules for days 1 through 28 under quiescent or osteogenic conditions on compliant or stiff substrates. N = 3, Scale bar = 500 µm. *p < 0.05 compared to corresponding quiescent media condition.
