Table 2 Primer Sequences for qRT-PCR.
From: Label-free metabolic biomarkers for assessing valve interstitial cell calcific progression
Target | Gene | Forward Sequence | Reverse Sequence |
|---|---|---|---|
αSMA47 | ACTA2 | 5′-CAGTTTTCCCTTCCATCGTG-3′ | 5′-TGGGGTATTTCAAGGTCAGG-3′ |
RhoA10 | RHOA | 5′-ACCAGTTCCCAGAGGTGTATGT-3′ | 5′-TTGGGACAGAAATGCTTGACTTC-3′ |
TGFβ Receptor126 | TGFβR1 | 5′- GACGGCATTCCAGTGTTTCT-3’ | 5′-TGCACATACAAATGGCCTGT-3′ |
Osteopontin4 | OPN | 5′-TTGCTAAAGCCTGACCCATCT-3′ | 5′-CGTCGTCCACATCGTCTGTT-3′ |
Osteocalcin4 | OCN | 5′-TCAACCCCGACTGCGACGAG-3′ | 5′-TTGGAGCAGCTGGGATGATGG-3′ |
RUNX242 | RUNX2 | 5′-GCACTACCCAGCCACCTTTA-3′ | 5′-TATGGAGTGCTGCTGGTCTG-3′ |
18S47 |  | 5′-ATTCCGATAACGAACGAGACT-3′ | 5′-GGACATCTAAGGGCATCACAG-3′ |
