Figure 2

Comparison of fold change of cytokine expressing MAIT and CD8⁺ non-MAIT cell subsets in blood. Fold change following PMA/Ionomycin stimulation of (A) TNF-α⁺, (B) IFN-γ⁺, (C) IL-17⁺, (D) Granzyme B⁺ and (E) Ki-67-expressing MAIT and CD8⁺ non-MAIT cells compared to pre-samples (naïve) over the course of vaccination and infection. Significant differences between cytokine-expressing MAIT and non-MAIT cells at indicated time points are also shown. Data of (A–E) were analyzed by the Wilcoxon paired test. Horizontal and vertical bars denote mean and SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. The black arrows indicate persistence of significant differences compared to the naïve sample up to the time point marked by the dashed line. For simplicity, p values for each comparison ranging from <0.05 to <0.0001 are not shown. Similarly, the blue arrows indicate continuing significant differences between MAIT and non-MAIT cells at sequential time points.