Table 1 Concentrations and incubation times for the hFRUIT clearing protocol.
From: hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue
Incubation time [days] | Urea [g/ml] | Fructose [g/ml] | Sucrose [g/ml] | Thioglycerol [g/ml] |
|---|---|---|---|---|
1 | 0.24 | 0.04 | 0.04 | 0.02 |
1 | 0.24 | 0.08 | 0.08 | 0.04 |
2 | 0.24 | 0.16 | 0.16 | 0.08 |
2 | 0.24 | 0.24 | 0.24 | 0.12 |
3 | 0.24 | 0.32 | 0.32 | 0.16 |
3 | 0.24 | 0.385 | 0.37 | 0.2 |
