Figure 4 | Scientific Reports

Figure 4

From: Lysophosphatidylcholine acyltransferase 2 (LPCAT2) co-localises with TLR4 and regulates macrophage inflammatory gene expression in response to LPS

Figure 4

LPCAT2 silencing reduces LPS-induced TNF-α gene expression and protein release from mouse peritoneal macrophages and a human monocyte cell line. LPCAT2 expression was significantly reduced by incubation of primary mouse peritoneal macrophages with LPCAT2 siRNA (p = 0.0192 vs control negative siRNA) (A). LPCAT2 silenced primary macrophages show a significant reduction in TNF-α gene expression (B) and protein release (C) following stimulation with LPS (100ug/ml). Transduction of the human monocytic cell line, MM6, with shRNA lentiviral particles targeting the LPCAT2 gene resulted in reduced production of TNF-α (D) and IL-6 (E) cytokines following LPS stimulation. *p < 0.05 vs negative siRNA (B,C) vs vector control (D,E).

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