Figure 6
From: Ghrelin inhibits autonomic response to gastric distension in rats by acting on vagal pathway
Gastric distension and administration protocol. (A) After anesthesia, a carotid pressure transducer and an intragastric balloon were implanted in rat. A first set of gastric distension ranging from 20 to 80 mmHg with 20 mmHg increments and 4 min resting intervals between distensions was performed. Each distension lasted 20 s and was repeated twice. After 15 min recovery atropine (1 mg/kg i.v.) was administered then saline or ghrelin (30 µg/kg i.v.). Five minutes after the injection a second set of gastric distension was performed. In the third experiment the Ghrelin receptor antagonist of [D-Lys3]-GHRP-6 (0.3 µg kg i.v.) was injected 10 minutes before ghrelin administration.
