Figure 1 | Scientific Reports

Figure 1

From: TET1 Deficiency Impairs Morphogen-free Differentiation of Human Embryonic Stem Cells to Neuroectoderm

Figure 1

CRISPR-mediated mutations of catalytic domain of TET1 in H9 hESCs. (a) Double strand break was introduced by CRISPR/Cas9 before the first iron binding site to induce non-homologous end joining in TET1 gene. (b) Sequencing traces of PCR products amplified from genomic DNA of two hESC clones (CDKO-1 and CDKO-2), showing frame shift mutations in both alleles in each clone. (c) Sequencing of cloned PCR products showing homozygous 1 bp deletion in CDKO-1 clone and compound heterozygous deletions of 1 bp and 2 bp in CDKO-2 clone. The frameshift mutations destroy iron-binding sites and downstream catalytic domain. (d) Normal karyotype for the two TET1 mutant clones.

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