Figure 3
Pluripotency maintained in TET1-deficient hESCs. (a–i) Phase contrast images (a-a”) and immunostaining of indicated pluripotency markers (b-h”) in wild-type (a–h) or TET1-deficient (a’-h”) H9 hESCs. Fluorescence intensity was quantified from at least five independent colonies for each condition and normalized against the values in WT (i). (j–m”) Wild-type (j–m) or TET1-deficient (j’-m”) H9 hESCs were differentiated spontaneously in serum-containing medium through embryoid bodies (j–j”) to cells of all three germ layers, as indicated by immunostaining for the ectoderm marker TUJ1 (k-k”), the mesoderm marker SMA (l-l”) and the endoderm marker AFP (m-m”). Blue bars, 100 µm; white bars, 25 µm.
