Scientific Reports

Table 3 Substrates used in screening for oxidation reaction of BPSS2242.

From: Functional analysis of BPSS2242 reveals its detoxification role in Burkholderia pseudomallei under salt stress

Type of substrate	Substrate	Enzyme activity (µM min⁻¹ mg⁻¹)
Monosaccharides	α-Glucose	1.14^a
	Galactose	0.34^a
	Fructose	0.82^a
	Xylose	3.33^a
	Mannose	na
Disaccharides	Maltose	na
	Sucrose	na
	Lactose	na
	Trehalose	na
	Maltotriose	na
Aliphatic alcohols	Methanol	na
	Ethanol	na
	1-Propanol	na
	1-Butanol	na
	1-Hexanol	na
	1-Octanol	na
Aromatic alcohol	Benzyl alcohol	na
Polyol	2, 3-Butanediol	na
Steroid	Testosterone	na
Aldehyde	Acetoin	na

The reaction mixture contained 20 mM Tris–HCl (pH 8.0), 500 µM NAD(P)⁺ and various concentrations of substrate (0.1–100 mM).
na no activity.
^aNAD⁺ was used as a cofactor.

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