Table 5 Sequences of primers, lenghts of PCR products/restriction fragments and corresponding restriction enzymes, used for PCR–RFLP procedures for investigation of selected FCN1 gene single nucleotide polymorphisms.
From: Associations of ficolins and mannose-binding lectin with acute myeloid leukaemia in adults
SNP | Primers (forward/reverse) | PCR product (bp) | Enzyme | Restriction fragments (bp) |
|---|---|---|---|---|
-542 G > A | 5′-CCCAGAAAATTCAGGGTTTG-3′ | 148 | Taq1 | 123 + 25 |
rs10120023 | 5′-TAACTTTCAAATAATTTACTCCATC-3′ | 65 °C, 24 h | ||
-144 C > A | 5′-TGAAGAGTCCCCCAGCTCT-3′ | 150 | BsuRI (HaeIII) | 130 + 20 |
rs10117466 | 5′-GGAAACATCCTTTGAGATGGC-3′ | 37 °C, 24 h | ||
+ 7,959 A > G | 5′-CACTAGCAGGTGCATGTGGA-3′ | 177 | Tru1I | 156 + 21 |
rs138055828 | 5′-CGACTGTCATGCTTCAAACCTTA-3′ | 65 °C, 24 h |
