Table 1 List of primary antibodies. (GFAP) Glial fibrillary acidic protein, (GFP) green fluorescent protein, (pFAK) phosphorylated FAK, (MBP) myelin basic protein, (ICC) immunocytochemistry, and (WB) western blot.
From: Secretion of a mammalian chondroitinase ABC aids glial integration at PNS/CNS boundaries
Antibody | Clonality | Isotype | Host | Concentration | Company |
|---|---|---|---|---|---|
1B5 | IgG | Monoclonal | Mouse | 1:500 (ICC) 1:1,000 (WB) | Seikagaku |
CS-56 | IgM | Monoclonal | Mouse | 1:300 (ICC) 1:1,000 (WB) | Sigma |
Laminin | IgG | Polyclonal | Rabbit | 1:300 (ICC) | Sigma |
βIII-tubulin | IgG | Polyclonal | Rabbit | 1:2000 (ICC) | Covance |
βIII-tubulin | IgG | Monoclonal | Mouse | 1:200 | ThermoFisher Scientific |
pFAK (try397) | IgG | Polyclonal | Rabbit | 1:100 (ICC) | Invitrogen |
FAK | IgG | Monoclonal | Mouse | 1:100 (ICC) | Millipore |
GFP | IgG | Polyclonal | Rabbit | 1:1,000 (ICC) | AbCam |
GFP | IgG | Polyclonal | Rabbit | 1:1,000 (ICC) | Life Technologies |
GFAP | IgG | Polyclonal | Rabbit | 1:500 (ICC) | DAKO |
GFAP | IgG | Monoclonal | Mouse | 1:300 (ICC) | Sigma |
GFAP | IgG | Monoclonal | Chicken | 1:2000 (ICC) | AbCam |
p75NTR | IgG | Monoclonal | Human | 1:50 (ICC) | Millipore |
p75NTR | IgG | Monoclonal | Mouse | 1:1,000 (ICC) | Millipore |
Aggrecan (Cat 301) | IgG | Monoclonal | Mouse | 1:300 (ICC) 1:500 (WB) | Millipore |
Aggrecan (AB1031) | IgG | Polyclonal | Rabbit | 1:300 (ICC) 1:500 (WB) | Millipore |
Ki67 (PP-67) | IgG | Monoclonal | Mouse | 1:200 (ICC) | AbCam |
MBP | IgG | Polyclonal | Rabbit | 1:1,000 (ICC) | AbCam |
MBP (G7G) | IgG | Monoclonal | Mouse | 1:2000 (ICC) | AbCam |
S100 | IgG | Monoclonal | Mouse | 1:100 (ICC) | ThermoFisher Scientific |
Hoechst-33342 | 1:10,000 (ICC) | Sigma | |||
Dappi (in ProLong Gold mounting medium) | ThermoFisher Scientific |
