Figure 7

Identification of TaMIPS-B lnteractors. (a) Western blot analysis of His-TaMIPS-8 recombinant protein with anti-His antibody. (b) Far-western blot analysis was done using 50ug of purified recombinant protein. After extensive washes, interacting protein were detected using anti-His antibody and visualized with lmmobilonTM western (Millipore). (c) Isolation of TaMIPS-8 interacting proteins from plant lysates by in-vitro pull-down assay. SDS_PAGE (12%) demonstrating, a putative interactors of TaMIPS-8 protein.