Figure 5 | Scientific Reports

Figure 5

From: Role of water in cyclooxygenase catalysis and design of anti-inflammatory agents targeting two sites of the enzyme

Figure 5

Experimental studies with wt- and mu-COX-2: (A) UV–vis spectra of T383A-H386V muCOX-2 reaction: (i) no AA, (iii) AA, (ii) after 5 min of AA addition. (B) schematic representation of blockage of H·/e transfer in T383A- H386V COX-2. (C) Change of enzymatic activity with temperature. 1–8 correspond to entries in Table 1. All values were average of 3 expts, SD ± 0.5–2.0 µM-1 s-1.

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