Figure 2

Subcellular locations of PhCS. PhCS C-terminal GFP fusion proteins were transiently expressed in petunia protoplasts coexpressing gRFP, mRFP and pRFP, which served as Golgi, mitochondrion and peroxisome markers, respectively, and visualized by confocal microscopy. (A,B) CS-GFP chloroplasts were visualized by autofluorescence. Chl, chlorophyll. (C) CS-GFP and RFP, (D) CS-GFP and mRFP, (E) CS-GFP and gRFP, and (F,G) CS-GFP and pRFP. Scale bars: 5 µm. Images were processed by Zen 2010 (version 6.0, Carl Zeiss Microscopy GmbH, Germany) software.