Figure 5
(a) Figure shows the strategy used to identify deregulated genes of lncRNA-GSE and lncRNA-CTD in L02 cells. LncRNA-GSE and lncRNA-CTD were transfected into L02 cells respectively. RNA sequencing was then performed on lncRNA-GSE overexpressing cells and lncRNA-CTD overexpressing cells to identify genes deregulated by lncRNA-GSE and lncRNA-CTD. Genes that are differentially expressed by |FC|≥ 2 in lncRNA-GSE overexpressing cells versus control or lncRNA-CTD overexpressing cells versus control were selected for further analysis. The differentially expressed genes that are correlated with lncRNA-GSE/lncRNA-CTD (|PCC|≥ 0.6) in the same direction in patient microarray dataset were included for pathway analysis. Red arrow: upregulated mRNAs; green arrow: Downregulated mRNAs. (b) Pathways of genes upregulated by lncRNA-GSE are mainly related to epigenetic mechanism, RNA polymerase I activity, cell division stage and regulation of rRNA expression. (c) Pathways of genes upregulated by lncRNA-CTD are involved in epigenetic mechanism, RNA polymerase I activity, Nonhomologous end joining, rRNA regulation, packaging of telomere ends and E3 ubiquitin ligases. Each line represents the common genes shared between the pathways. (d) Pathways of genes downregulated by lncRNA-GSE are related to carboxylic acid metabolism, proteoglycan metabolism, aclyglycerol biosynthetic process, regulation of cellular carbohydrates and serine-type endopeptidase. (e) Pathways of genes downregulated by lncRNA-CTD are mainly involved in amino acids, starch and sucrose, fatty acids and drug metabolism.
