Table 4 Sequences of primers and PCR condition.
Genes | Exon | Forward | Reverse | Hybridation temp. (°C) |
|---|---|---|---|---|
RPGRIP1 | 19 | AAAGAAGGCAGGAAGGAAGG | TCTTGAAAGCCTGATCTCGTG | 58 (bet) |
GUCY2D | 14 | GACCGGCTGCTTACACAGAT | GACAGGAGGTCTGGGAAAGA | 58 |
RPE65 | 7 | GCCTGTATAAGCTGTTCTAA | CTCAGTTACAAGAATCAACAG | 60 |
IQCB1 | 11 | AGATTGCACAACAGCAGCAG | CAGAGAAAAAGGACAAAAGTCCA | 60 |
CRYBA1 | 5 | TTTCTCACAAATCTGTTGCCTTA | CCGATACGT ATGAAATC TGATTAAAA | 58 |
CRB1 | 10 | CCTCCAGCAGGAGCTTTTTA | GCATAGATTTTCCTATGGGAACTG | 60 |
CRB1 | 7bb | GCTGACTCCAAACTCTCCCA | TGGTGGGTCAGTAACATGATCT | 60 |
CRB1 | 6b | GCAACAGGGATGTGTTTGTG | TTTCATAGCAGGCAGAAGCA | 65 |
CDHR1 | 10 | GGGAGCTGGACAGAAGTGAG | CACCTCCTCTTGCCTTTCTG | 58 |
C8ORF37 | 5 | CAGTAATCTGTAATATGTGGTGTATCC | CCCACAAGATCTGGCTGAAT | 58 |
C2ORF71 | 1E | GCAGCAAGTCCACAGAGAAC | TGTAAGAGGAGGGAAGGCTC | 58 |
ABCA4 | 13 | GGTGAGAGTCTGATACCTCT | AGCCAACTCGAAATGGCTCT | 58 (bet) |
ABCA4 | 28 | GGCTTGACTACTTCCATAGC | AGGTTACATGGACCTCAGCT | 58 (bet) |
ABCA4 | 9 | TCCATGGAAGCAGTGACTTTT | CAATGTCACTCATTATCTTCAGCA | 58 |
ABCA4 | 22 | ATACGTGACCATGGAGCTTG | AACAAGCTCATCTGACCAGG | 58 |
NMNAT1 | 2 | TGGCAGAGCAAGACCTTATC | GGACTACAGGCACAGTGAAT | 60 |
PRPH2 | 1 | TCGTTAAGGTTTGGGGTGGGA | CTGGTCAGAGGCCTGAGCCT | 58 |
FAM161A | 3 | TGGTCACATACAACTGAAAGTATAAATAACA | GCTTCTGTTCCTCCCTTGCT | 60 |
CNGB1 | 23 | AGAGACTCCGCCTCTCACTC | GGGGCAGACACGAAGATG | 58 |
EYS | 29 | AATCTGCTTCTGGCTTTGTTT | GCCCCACTAGCCAGAAAATA | 58 |
EYS | 12 | TTTTTAAATGCACCCCACAA | ACCAATCAATAGACACATTTGAGA | 58 |
BBS5 | 4 | AGGAGACAGAATTGACCCTCT | CATGAAACTGGTCCCTGGTG | 58 |
BBS5 | 2 | AAATGCATGAACATTTGGTACA | CACAATTACACTGACAAATGATGC | 58 |
CNGA3 | 8A | GCTGTGGCAGCATTACAAGA | GAATCAATCTTGGCCTGGAA | 58 |
CNGB3 | 16 | CACCTGGACCCTCACCTCTA | CGGTTCTCCCTATCTCAGAGT | 58 |
TRPM1 | 27A | TTCTGAAAAATCACATAGCAATGAC | CGTTTCCACTGTTAGCTGAGTG | 58 |
