Figure 2 | Scientific Reports

Figure 2

From: Automated spheroid generation, drug application and efficacy screening using a deep learning classification: a feasibility study

Figure 2

Spheroid integrity is affected by the addition of cytotoxic drugs. (A) Representative images of spheroids consisting of mIMCD3 cells that are either labelled ‘unaffected’ (left image, > 80% living cells), ‘mildly affected’ (middle image, 40–60% viability) or ‘affected’ (right image, < 40% viability. Size marker = 50 µm. (B) Corresponding flow cytometry analysis to images presented in (A). Cell viability was assessed by flow cytometry using Annexin-V counterstain. (C) Viability of spheroids consisting of mIMCD3 cells exposed for 24 h to the pan kinase inhibitor Staurosporine in the indicated concentrations. N = 4–9, p < 0.001 for nt against both treated conditions. (D) Viability of spheroids consisting of mIMCD3 cells exposed for 24 h to the topoisomerase inhibitor Etoposide in the indicated concentrations. N = 7–9, p < 0.001 for nt against both treated conditions.

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