Figure 4 | Scientific Reports

Figure 4

From: Automated spheroid generation, drug application and efficacy screening using a deep learning classification: a feasibility study

Figure 4

Proposed workflow to optimize drug screening in solid tumours. (1) A malignant lesion grows in vivo. (2) Primary cells are secured by a biopsy and (3) expanded to yield a sufficient number of cells in vitro. (4) Homogeneous spheroids are generated via the hanging drop method and (5) are incubated until spheroids are fully matured. (6) Combinations of different drugs and concentrations are applied and the response of each spheroid is tracked via imaging. (7) Images are analysed by a pre-trained CNN and (8) allow a classification regarding the sensitivity towards a specific drug or concentration.

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