Figure 6
From: The cellular heat shock response monitored by chemical exchange saturation transfer MRI
(a) Temperature and T1 time course during heat shock of 42 °C. Temperature was assessed by an infrared thermometer by placing the bioreactor with collagenized MCAs in medium on the animal bed in front of the MRI. The T1 time course is from the heat shock experiment of cell culture 1. The heating up to 42 °C and the cooling down to 37 °C took approximately 15 min, while cells were exposed to a heat shock of 42 °C for 20 min. The change in T1 correlates well with the observed temperature change. After the 15 min cool down, the T1 value was constant showing that the rNOE signal changes could not be explained by a recovery of T1. (b) Sequence and heat bath timing of the heat shock protocol. T1 was finer sampled around the heat shock of 42 °C. For a fast heating up to 42 °C, the second heat bath was preheated, while a rapid cooling to 37 °C was achieved by addition of ice-cold water to both heat baths.
