Figure 5

Concentration-dependent effects of IFX (in PBST) on the SPR wavelength shifts (Δλ) using an SPR-POF surface-functionalized with the anti-IFX antibody. The experimental data were fitted by non-linear regression using the ‘one-site’ binding model, i.e. the simplest model describing the equilibrium binding of a ligand (IFX) to a receptor (anti-IFX antibody) as a function of increasing ligand concentration. The best-fit values of the model parameters (Bmax and Kd), are indicated with their 95% confidence interval values (in parenthesis). The error bars correspond to the upper bound of the experimentally measured standard deviation.