Table 1 Primers used in qRT-PCR primer assays.
From: Laminin-511-E8 promotes efficient in vitro expansion of human limbal melanocytes
Gene symbol | Accession no | Product length | Probe no./SYBR | Sequence 5′–3′ |
|---|---|---|---|---|
GAPDH | NM_002046.3 | 72 | P3 | CAGCAAGAGCACAAGAGGAA |
GTGGTGGGGGACTGAGTGT | ||||
KIT | NM_000222.2 | 61 | P6 | GAGTAGCTTACCAGAAGCTTCCATAG |
CATAGGGACTGATGCCTTCC | ||||
KRT3 | NM_057088.2 | 92 | P74 | CCTGTGATTGTCCAGGTGTG |
ATACATCAGAGCTGTAGTGAGCATC | ||||
KRT15 | NM_002275.3 | 60 | P10 | CATTGGCATCAGGGAAGC |
TTGATGTGGAAATTGCTGCT | ||||
LAMA1 | NM_005559.3 | 60 | P38 | AGGATGACCTCCATTCTGACTT |
CCTTACATGGGCACTGACCT | ||||
LAMA2 | NM_000426.3 | 68 | P81 | GCAAGCCACTGGAGGTTAAT |
TGGGCATGATACAGGTTGAA | ||||
LAMA3 | NM_198129.1 | 65 | P29 | CCAGGAATATGGGTTGCTTG |
GGGAGCAGCACCAGGTAAT | ||||
LAMA4 | NM_001105209.1 | 68 | P55 | GCCACACTCGTCCTTCTCTC |
CCCAGGTGAAACTCTCAAGG | ||||
LAMA5 | NM_005560.3 | 75 | P44 | ATGACTCGCTCTGTGGAGGT |
GGGGTTGGCTGTGTCCTA | ||||
LAMB1 | NM_002291.2 | 72 | P82 | AAGCCAGAAAGTTGCTGTGTATAG |
CCTTGTTCACCTCAGCCATT | ||||
LAMB2 | NM_002292.3 | 100 | P43 | AAGGCCTACCCCAGTTCCTA |
GGGTTCACACTGGTTTATTGG | ||||
LAMB3 | NM_001127641.1 | 72 | P23 | GGCATGCCATTGAAACTAAGA |
AGAACTAAAGGCGGGGGATA | ||||
LAMB4 | NM_007356.2 | 67 | P5 | CCCCACACCCTGTCCTTATT |
ATTTTCCTGGTGGCATTTCA | ||||
LAMC1 | NM_002293.3 | 92 | P66 | GCCATTATTTTATTGTCTAGCTCCA |
ATCCCTGTGTCAACCAGCAT | ||||
LAMC2 | NM_005562.2 | 96 | P65 | CACTCTGTGCCTTTCTACAACTG |
CCAAGGTGGAAGTGCCTCT | ||||
LAMC3 | NM_006059.3 | 67 | P14 | CAGGACTCCTCAGCATTTCC |
TTGCCATCTGCTGGAAGAG | ||||
MLANA | NM_005511.1 | 79 | P39 | GAGAAAAACTGTGAACCTGTGGT |
AAGGTGGTGGTGACTGTTCTG | ||||
NT5E | NM_002526.4 | 111 | P80 | CCAGTCCACTGGAGAGTTCC |
CGACACTTGGTGCAAAGAAC | ||||
TYRP1 | NM_000550.2 | 77 | P2 | CCTGTGACCAGAGGGTTCTC |
CCGGACAAAGTGGTTCTTTTC |
