Figure 4
Lesion distribution through TA regions. Mouse TAs were electroporated with different doses of pCIneo-DUX4 (1, 5,10, 20 and 40 µg) and 10 µg of pCMV-lacZ. Muscle damaged area 1 week following TA hIMEP was evaluated in the injected area (β-gal+) of HEB stained cryosections from distal, medial and proximal regions of TA (color thresholding, ImageJ). Since no statistical difference was observed between tested doses considering each region individually, data were respectively pooled to form single proximal, medial and distal groups (data not shown, One-way Anova, p = 0.871, 0.821 and 0.395 in proximal, medial and distal region respectively). All data are presented as boxplots, *p < 0.05 and **p < 0.01. Friedman Repeated Measures Analysis of Variance on Ranks; n = 15 for each group. Graphs were generated using GraphPad Prism 6.01.
