Figure 7
Confirmation of DUX4 protein expression by Immunofluorescence. Mouse TAs were injected by hIMEP with 10 µg pCMV-lacZ plasmid concomitantly with 40 µg either pCIneo-DUX4 or pCIneo, or with a saline solution (negative control), as indicated. Cryosections were analyzed 1, 3 and 7 days post-injection by immunofluorescence with antibodies directed against either DUX4 (E5-5) (red) or laminin α2 (green) to stain the myofibre basal lamina (for details, see “Methods”). DAPI was used to visualize nuclei (blue). Pictures were taken with a Nikon Eclipse 80i microscope and merged using NIS-Elements software. Scale bar 50 µm.
