Figure 1
From: SimpylCellCounter: an automated solution for quantifying cells in brain tissue
Schematic of automated processing steps. (A) Selected parameters for threshold (pixel intensity), object size (pixel radius) and object circularity for Manual, OpenColonyFormingUnit (OCFU), ImageJ Edge Detection Macro (IMJM) and SimpylCellCounter (SCC) of cFos-immunoreactive (cFos-IR) cells. (B) Image processing sequence for SCC: (1) loads images and converts to 8-bit grayscale making all pixel intensities range between 0 and 255, (2) performs global threshold based on a user-set value and creates a binarized mask, (3) performs morphological operations on the binary mask to filter out noise-like particles, (4) further selects for cells based on size, circularity and overlap, resulting in a final cell count.
