Figure 4

Performance Analytics of Automated Methods. OpenColonyFormingUnit (OCFU, orange), ImageJ Edge Detection Macro (IMJM, gray) and SimpylCellCounter (SCC, blue) performance analytics were compared. (A) Average time (sec) to quantify cFos-Immunoreactive (IR) cells per image (1920 × 1460 pixels), n = 30 images per automated method. (B) Average time (sec) as a function of image size (pixels) to quantify the number of cFos-IR cells per image, n = 30 images per automated method. Inset displays data for SCC only. (C) Average absolute error per image where: \(ABS\left(Manual\;counts-Automated\;counts\right)\) as the binary threshold value approaches the mean pixel intensity of the image, n = 15 images per threshold factor. Inset displays data for SCC only.