Figure 2

The specific composition of soluble Aβ in nasal discharges from different stages of probable AD group (pAD). (A) The total levels of soluble Aβ species using CLASS method (self-standard ratio (a.u.)) in nasal discharges were measured in (Normal (22), mild stage (25) and moderate stage (13) of pAD groups. (B) Quantification of soluble Aβ*56 + AβO protein levels. Expression levels of proteins were quantified using stereological analysis (ImageJ program) in Normal (18), mild stage (22) and moderate stage (17) of pAD groups. (C) Quantification of soluble Aβ*56 protein. Expression levels of proteins were quantified using stereological analysis (ImageJ program) in Normal (18), mild stage (22) and moderate stage (17) of pAD groups. (D) Quantification of soluble AβO protein. Expression levels of proteins were quantified using stereological analysis (ImageJ program) in Normal (12), mild stage (9) and moderate stage (10) of pAD groups. Data are represented as means ± SEMs from three independent experiments. Value of samples were identified as outliers through Grubbs’ test, also called the ESD method. For the statistical analysis, one-way ANOVA was performed, followed by Dunnett’s post hoc test. Statistical significance is denoted (ns > 0.05, ***P < 0.001). In addition, paired t-test was performed. Statistical significances are denoted (ns > 0.05, ^#P < 0.05, ^##P < 0.01, ^###P < 0.001).