Figure 3

Experimental trabeculectomy with SPG-178. (a) Time course of intraocular pressure (IOP) change after a trabeculectomy operation in the SM group (black circles), M group (black squares), and control C group (black triangles). The IOP was significantly lower in the SM and M groups compared to IOP in the C group between 7 and 28 days (p < 0.001 by ANOVA, **p < 0.01; and n.s., not significant by Dunnett’s test). (b, c) A bleb (white arrow) was formed at postoperative day 28 in the SM, M, and C groups as seen by both slit-lamp observation (b) and optical coherence tomography (c). (d) The height of the bleb at postoperative day 28 was significantly greater in the SM and M groups compared to the C group (p = 0.004 by ANOVA, **p < 0.01; and n.s., not significant by Dunnett’s test). Error bars represent SDs [n = 6 (C group), n = 5 (M and SM groups)]. (e) Histology of the sclera on postoperative day 28 in the SM and M groups. The arrowhead indicates the cornea, the arrow the iridectomy wound, the dotted arrow the scleral flap, and the dotted line the bleb. Scale bar = 1 mm. (f, g) Slit lamp observation after injection of SPG-178 into the anterior chamber. The SPG-178 became cloudy, but the cornea remained clear. (f) 4 h after its insertion, (g) 2 weeks after the operation. The white arrowhead indicates cloudy SPG-178 adhered to the corneal endothelial surface.