Figure 6 | Scientific Reports

Figure 6

From: A new functional JAZ degron sequence in strawberry JAZ1 revealed by structural and interaction studies on the COI1–JA-Ile/COR–JAZs complexes

Figure 6

Yeast two-hybrid (Y2H) assays for JAZs-COI1s interactions using Fragaria × ananassa and Arabidopsis thaliana proteins. Interactions of FaCOI1 (a) and AtCOI1 (b) with JAZs repressors of Fragaria × ananassa and Arabidopsis thaliana under absence or presence of COR. Negative controls (c) with pGADT7-(AD) and pGBKT7-(DBD) empty vectors under the absence/presence of COR. − 2, SD-Leu-Trp; − 3, SD-Leu-Trp-His; − 4, SD-Leu-Trp-His-Ade; At, Arabidopsis thaliana; Fa, Fragaria × ananassa; COI1, CORONATINE INSENSITIVE1; COR, coronatine; JAZ, JASMONATE ZIM-DOMAIN.

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