Figure 8 | Scientific Reports

Figure 8

From: A new functional JAZ degron sequence in strawberry JAZ1 revealed by structural and interaction studies on the COI1–JA-Ile/COR–JAZs complexes

Figure 8

Yeast two-hybrid (Y2H) assays for Fragaria × ananassa JAZ1 mutants- and chimeras-COI1 interactions. Interactions of FaCOI1 with native (FaJAZ1), single (FaJAZ1_AK and FaJAZ1_RA) and double (FaJAZ1_AA) mutants, and chimeras (Fa/AtJAZ1 and At/FaJAZ1) of JAZ1 under absence/presence of COR. Negative controls with pGADT7-(AD) and pGBKT7-(DBD) empty vectors under the absence/presence of COR. − 2, SD-Leu-Trp; − 3, SD-Leu-Trp-His; − 4, SD-Leu-Trp-His-Ade; Fa, Fragaria × ananassa; COI1, CORONATINE INSENSITIVE1; COR, coronatine; JAZ, JASMONATE ZIM-DOMAIN.

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