Figure 9

Representation of main FaCOI1–FaJAZ interactions with different JAZ degrons by the yeast two-hybrid (Y2H) system in the presence of COR performed in the present research. FaCOI1 showed strong interactions with IPMQRK (FaJAZ1) and VPQARK (AtJAZ9) degron sequences, weak and no interaction with LPIARR (FaJAZ10 and AtJAZ1, respectively) degron, and no interaction with FaJAZ8.1 and AtJAZ8 (a). In turn, AtCOI1 interacts strongly both with IPMQRK (FaJAZ1) and LPIARR (FaJAZ10) (a). When tested single and double mutants and chimeras of FaJAZ1 degron (b) the FaCOI1–COR–FaJAZ1 is not formed in the double mutant (FaJAZ1_AA) but a weak interaction is produced with the FaJAZ1 degron variants IPMQAK, IPMQRA, IPMARR, and LPIQRK, indicating a crucial role of Arg and Lys residues and a minor role of Ile-Pro-Met sequence of the FaJAZ1 degron in the formation of FaCOI1–COR–FaJAZ1 complex. At, Arabidopsis thaliana; Fa, Fragaria × ananassa; COI1, CORONATINE INSENSITIVE1; COR, coronatine; JAZ, JASMONATE ZIM-DOMAIN; CMID, Cryptic MYC2-interacting domain; EAR, ethylene-responsive element binding factor-associated amphiphilic repression; TIFY, TIFY domain.