Table 2 List of oligonucleotides.
Target gene | Primer | Purpose | Nucleotide sequences | Source |
|---|---|---|---|---|
IL-6 | F | RT qPCR | GCAGAAAAAGGCAAAGAATC | Sigma-Aldrich |
R | CTACATTTGCCGAAGAGC | |||
IL-8 | F | RT qPCR | GTTTTTGAAGAGGGCTGAG | Sigma-Aldrich |
R | TTTGCTTGAAGTTTCACTGG | |||
MMP9 | F | RT qPCR | AAGGATGGGAAGTACTGG | Sigma-Aldrich |
R | GCCCAGAGAAGAAGAAAAG | |||
TRAF1 | F | RT qPCR | CTTTCCTGTGGAAGATCAC | Sigma-Aldrich |
R | ACTTGGCAGTGTAGAAGG | |||
ICAM1 | F | RT qPCR | ACCATCTACAGCTTTCCG | Sigma-Aldrich |
R | TCACACTTCACTGTCACC | |||
GAPDH | F | RT qPCR | CTTTTGCGTCGCCAG | Sigma-Aldrich |
R | TTGATGGCAACAATATCCAC | |||
TFRC | F | RT qPCR | AAGATTCAGGTCAAAGACAG | Sigma-Aldrich |
R | CTTACTATACGCCACATAACC | |||
IL-6-prom | F | Promoter PCR | CCGGGTACCTCCAAGGCAGACTCTGAG | Sigma-Aldrich |
R | GGCCAAGCTTCATCTCCAGTCCTATATTTATTGGGGG | |||
M13 | R | PCR | CAGGAAACAGCTATGAC | Sigma-Aldrich |
SRC-3-A | F | PCR | AGGAAGGGGAAGGTAAGAGCTA | Sigma-Aldrich |
R | CACAGGGTTTGATGGAAATGTT | |||
SRC-3-B | F | CRISPR sgRNA | GCAATCTTGTATGATCTGTG | Life Technologies |
R | CACAGATCATACAAGATTGC | |||
SRC-3-C | F | siRNA | CAGUAUAUCGAUUCUCGUUtt | Ambion life technologies |
R | AACGAGAAUCGAUAUACUGgg | |||
SRC-3siR | F | Mutagenesis | CCATGCAGAAACCCCCGTCTACCGCTTCTCGTTGGCTGAT | Sigma Aldrich |
R | ATCAGCCAACGAGAAGCGGTAGACGGGGGTTTCTGCATGG | |||
IKK-α | F | siRNA | GAAGGAUCCAAAGUGUAUAtt | ThermoFisher Scientific |
UAUACACUUUGGAUCCUUCgg |
