Figure 5
The P1 and P1′ amino acid sites of NS2A/2B strongly affect DTMUV replication. (A) Schematic of the construction of DTMUV mutation replicons. (B) BHK21 cells were transfected with the replicons and harvested at 36, 48 and 60 h post transfection. Then, the replication of DTMUVmut replicons was evaluated by measuring the Renillaluciferase level, CQW1-Rep-Rluc-GDD/AAA was used as negative control. Significant differences from the mock groups are indicated by *P < 0.05, **P < 0.01 and ***P < 0.001.
