Figure 6
The P1 and P1′amino acidsites of NS2A/2B are important for P0 DTMUV proliferation. (A) Schematic of the recombinant viruses rescue and data collection. (B) IFA detection of rDTMUV-WT and rDTMUV-NS2A/2B-P1P1′(AA). BHK21 cells were transfected with rDTMUV RNA, and the cells were harvested 1, 3, 5, 8 days post transfection for IFA, and mouse anti-DMTUV polyclonal antibody was used as the primary antibody. (C) Quantitative annals of IFA data in Fig B. (D) Plaque assay for rDTMUV-WT and rDTMUV-NS2A/2B-P1P1′(AA). rDTMUV-WT caused larger plaque sizes than rDTMUV-NS2A/2B-P1P1′(AA) at 4 days post transfection. (E) Genome copy numbers of both intracellular and extracellular rDTMUV-WT and rDTMUV-NS2AB-P1P1′(AA) samples. (F) Growth curves of both intracellular and extracellular rDTMUV-WT and rDTMUV-NS2AB-P1P1′(AA) samples. The viral genome copy number and TCID50 of each sample were measured at different time points. All data are represented as the mean ± SEM (n = 4). Significant differences from the mock groups are indicated by *P < 0.05, **P < 0.01 and ***P < 0.001.
