Figure 8
From: Region-specific mechanisms of corticosteroid-mediated inotropy in rat cardiomyocytes
Effect of DI treatment on SERCA and PLB protein expression and phosphorylation. (A-D) Representative western blots stained for SERCA2 (A, 100 kDa), PLB (B, 25 kDa), phospho-PLB-Ser16 (C, 25 kDa) and phospho-PLB-Thr17 (D, 25 kDa). Protein was isolated from left ventricular myocytes incubated for 24 h under control conditions (Control) or with DI. Arrows indicate the according molecular weight level of the target protein. (E) Average relative protein expression was assessed by western blot quantification (normalization by Ponceau staining) of control (blue) and DI treated myocytes (green). n = 5 matched cell isolations. The full length gels of the western blots including the corresponding Ponceau stains are shown in the supplemental figure.
