Figure 6

Chronological expression of nodal markers in human ES cell-derived cardiomyocytes (hESC-CMs) on three-dimensional cultures. hESC-CMs were thawed to form cardiac spheroids and cultured. Histological analysis was performed at the same time-points. (a–c) HCN4 (red) and cTNT (green). (d–f) SHOX2 (red) and cTNT (green). (g–i) TBX3 (red) and cTNT (green). (j,k) Fractions of SHOX2+ and TBX3+ cardiomyocytes, respectively (n = 3 per group). Data represent mean ± SEM.