Figure 5

Iloprost-induced changed in phosphoproteome of platelets from patients with suspected PHP Ia. (A) Heatmap of 453 platelet phosphopeptides identified as increased (green) or decreased (red) at indicated iloprost concentration per patient in comparison to mean effect in 3 or 4 control subjects (C1–4, C’). Left lane: mean effect of 10 nM iloprost in control platelets (see Fig. 4A). (B) Heatmap as in panel A, but restricted to relevant differences, filtered for outside control range of log2 2× (− 0.322 to + 0.322). (C) Average ratios of iloprost effects in platelets from selected patients (P1–4,6) versus control subjects (C1–4) regarding top-100 up-, non-, and downregulated phosphopeptides.*p < 0.05 vs. controls (2-sided t test).