Figure 5 | Scientific Reports

Figure 5

From: Early modulation of macrophage ROS-PPARγ-NF-κB signalling by sonodynamic therapy attenuates neointimal hyperplasia in rabbits

Figure 5

ALA-SDT inhibits NF-κB signalling and subsequently suppresses the expression and secretion of TNF-α, IL-6 and IL-1β in THP-1-derived macrophages. Western blot analysis of the nuclear protein expression of NF-κB p65 (A) and the cytoplasmic protein expression of NF-κB p65 (B) and p-IκBα (C). Histone H3 and β-actin were used as loading controls for nuclear proteins and cytoplasmic proteins, respectively. (D) Immunofluorescence analysis of NF-κB p65 nuclear translocation. Scale bar = 20 μm. (E) Western blot analysis of p-p65 levels. (F) Quantitative real-time polymerase chain reaction analysis of mRNA levels of TNF-α, IL-6 and IL-1β. (G) Western blot analysis of TNF-α, IL-6 and IL-1β. (H) ELISA analysis of TNF-α, IL-6 and IL-1β in supernatants (n = 5). *p < 0.05, **p < 0.01 and ***p < 0.001 versus control.

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