Figure 4

Association of heart rate variability and heart rate at 2- and 5-days post-fertilization (dpf) and effect of nonsense mutations in both hcn4 alleles. Top: the association of heart rate variability and heart rate at 2 and 5dpf, with embryos carrying nonsense mutations in both hcn4 alleles shown as pink diamonds. Bottom: the mean ± standard deviation of heart rate variability and heart rate at 2dpf and 5dpf for embryos with nonsense mutations in both hcn4 alleles (n = 5) vs. embryos without CRISPR/Cas9-induced mutations in hcn4 (n = 147).