Figure 4

Analysis of autophagy-modulating compounds using eGFP-LC3B expressing cell lines. The eGFP-LC3B MFI of saponin-treated eGFP-LC3B expressing HeLa (A), HEK293T (B), Jurkat (C), or THP-1 (D) cells was quantified using flow cytometry and the background (= mock) subtracted. Cells were either mock-treated or treated with Rapamycin (2 µM—15 nM; left panel), Chloroquine (20 µM–150 nM; middle panel), or Bafilomycin A1 (625 nM–5 nM, right panel) for 4 h. Z-factors are indicated next to the respective diagram. The red dotted line indicates the double standard deviation of the mock control. Data are shown as mean(n = 3) ± SEM. (E) Background subtracted eGFP-LC3B MFIs of HeLa GL cells either mock-treated, or treated with 18 different human amino acids (1 mg/ml, as indicated in three-letter code), Rapamycin (1 µM), or Chloroquine (10 µM) for 4 h. Data are shown as mean(n = 3) ± SEM.