Table 2 Phusion HF (Thermo Scientific) pipetting scheme.
From: An improved method for high-throughput quantification of autophagy in mammalian cells
| Â | Volume per tube |
|---|---|
5 × Phusion HF Buffer | 10 µl |
10 mM dNTPs | 1 µl |
fwd Primer (10 pmol/µl) | 1 µl |
rev Primer (10 pmol/µl) | 1 µl |
Template DNA (0.1 µg/µl) | 1 µl |
Dimethyl sulfoxide | 1.5 µl |
Phusion HF DNA Polymerase | 0.5 µl |
ad 50 µl ddH2O |  |
