Table 3 Phusion HF PCR conditions.
From: An improved method for high-throughput quantification of autophagy in mammalian cells
| Â | Temperature | Duration |
|---|---|---|
Initial denaturation | 98 °C | 30 s |
35 amplification cycles | 98 °C | 5–10 s |
55 °C (adjust according to primer properties) | 10–30 s | |
72 °C | 15–30 s/kb | |
Final extension | 72 °C | 10 min |
Hold for storage | 4 °C | ∞ |
