Table 5 Materials used for high throughput quantification of autophagy.
From: An improved method for high-throughput quantification of autophagy in mammalian cells
Product | Source | Product number |
|---|---|---|
InSolution Rapamycin | Merck | 553211 |
Chloroquine diphosphate salt | Santa Cruz Biotechnology | sc-205629 |
Bafilomycin A1 | Santa Cruz Biotechnology | sc-201550 |
Dimethyl sulfoxide | Merck | 102952 |
PBS, no calcium, no magnesium | Gibco | 14190094 |
Trypsin 0.05%/EDTA 0.02% in PBS, w/o: Ca and Mg | PAN-Biotech | P10-023100 |
Saponin | Sigma-Aldrich | 47036 |
Paraformaldehyde solution 4% in PBS | Santa Cruz Biotechnology | sc-281692 |
Media | ||
RPMI 1640 Medium | Gibco | 21875034 |
Gibco DMEM, high glucose, no glutamine | Gibco | 11500416 |
Fetal Bovine Serum, qualified, Brazil | Gibco | 10270106 |
Penicillin–Streptomycin, 10,000 U/ml Penicillin, 10 mg/ml Streptomycin | PAN-Biotech | P06-07050 |
L-Glutamine 200Â mM | PAN-Biotech | P04-80050 |
