Table 6 Materials used for application examples of the eGFP-LC3B reporter system.
From: An improved method for high-throughput quantification of autophagy in mammalian cells
Product | Source | Product number |
|---|---|---|
InSolution Rapamycin | Merck | 553211 |
Chloroquine diphosphate salt | Santa Cruz Biotechnology | sc-205629 |
Bafilomycin A1 | Santa Cruz Biotechnology | sc-201550 |
Dimethyl sulfoxide | Merck | 102952 |
Polyethylenimine, branched | Sigma-Aldrich | 408727 |
Opti-MEM I Reduced Serum Medium | Gibco | 31985047 |
IAV, PR8 | ATCC | VR-95 |
EMCV, EMC | ATCC | VR-129B |
MeV, Schwarz strain | K.-K. Conzelmann | |
Paraformaldehyde solution 4% in PBS | Santa Cruz Biotechnology | sc-281692 |
Amino Acids | Sigma | various |
Methanol | Merck | 32213 |
Human CRISPR Knockout Pooled library (GeCKO v2) (39) | Addgene | #1000000048 |
NEBNext High-Fidelity 2X PCR Master Mix | New England Biolabs | M0541 |
Monarch PCR & DNA Cleanup Kit | New England Biolabs | T1030 |
UltraPure DNase/RNase-Free Distilled Water | Invitrogen | 10977 |
Quick-DNA Midiprep Plus Kit | Zymo Research | D4075 |
Puromycin Dihydrochloride | Gibco | A1113803 |
PBS, no calcium, no magnesium | Gibco | 14190094 |
Falcon Round-Bottom Tubes with Cell Strainer Cap, 5Â mL | Falcon | 38030 |
Saponin | Sigma-Aldrich | 47036 |
Media | ||
RPMI 1640 Medium | Gibco | 21875034 |
Gibco DMEM, high glucose, no glutamine | Gibco | 11500416 |
Fetal Bovine Serum, qualified, Brazil | Gibco | 10270106 |
Penicillin–Streptomycin, 10,000 U/ml Penicillin, 10 mg/ml Streptomycin | PAN-Biotech | P06-07050 |
l-Glutamine 200Â mM | PAN-Biotech | P04-80050 |
