Table 8 PCR protocol.
From: An improved method for high-throughput quantification of autophagy in mammalian cells
Step | Cycle # | Temperature (°C) | Duration |
|---|---|---|---|
Initial denaturization | 1 | 98 | 3Â min |
Denaturization | 2–29 | 98 | 10 s |
Primer annealing | 63 | 10Â s | |
Extension | 72 | 25Â s | |
Final extension | 30 | 72 | 2Â min |
