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Figure 1

From: Increasing endogenous activity of NMDARs on GABAergic neurons increases inhibition, alters sensory processing and prevents noise-induced tinnitus

Figure 1

M-8324 enhances spontaneous spiking of GABAergic neurons in vitro. (A) Sample traces showing 30 µM M-8324’s effect on NMDAR EPSCs in inhibitory neurons (Int) and excitatory neurons (Ext). Scale bars, 100 ms, 50 pA. (B) Population results on normalized EPSC peak and area during bath application of M-8324 (30 µM) for Ext and Int neurons. N = 6 cells/3 mice for each group. Normalized isolated NMDAR EPSC amplitude (C) and area (D) before and after bath perfusion of GNE-8324 or M-8324 in GABAergic neurons. N = 12 cells/4 mice for GNE-8324, N = 11 cells/4 mice for M-8324. (E) Sample recording showing no impact of M-8324 on AMPAR-EPSPs in GABAergic neurons. Scale bars, 50 ms, 2 mV. (F) Sample voltage traces showing M-8324′s effect on spontaneous spiking in GABAergic neurons in whole-cell recording mode. Scale bars, 4 s, 10 mV. (G) Normalized spontaneous spiking frequency during either M-8324 or Veh bath application in GABAergic neurons in AI slice. N = 8, 5 cells/3 mice for M-8324 and Veh, unpaired t test. (H) Frequency of spontaneous spiking before and after M-8324 application in the same neurons. Dotted line represent slope of 1 (no change). Data are presented as the mean ± SEM.

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